|
KMID : 0387420140250041423
|
|
Korean Society of Oriental Neuropsychiatry
2014 Volume.25 No. 4 p.1423 ~ p.434
|
|
|
Anti-inflammatory Effects of Cheongnoimyungshin-hwan in Microglia Cells
|
|
Im Yong-Gyun
Choi Yung-Hyun
Hwang Won-Deok
|
|
|
Abstract
|
|
|
Objectives: Activated microglia cells play an important role in inflammatory responses in the central nervous system (CNS) which are involved in neurodegenerative diseases. We attempted to determine the anti-inflammatory effects of Cheongnoimyungshin-hwan (CNMSH) in microglia cells.
Methods: We examined the effect of CNMSH on the inflammatory responses in BV2 microglia cells induced by lipopolysaccharide (LPS) and explored the mechanism underlying the action of CNMSH.
Results: BV2 cells treated with LPS showed an up-regulation of nitric oxide (NO), prostaglandin PGE2 (PGE2) and interleukin 1? (IL-1?) release, whereas CNMSH suppressed this up-regulation. CNMSH inhibited the induction of COX-2, iNOS and IL-1? proteins in LPS-treated BV2 cells and blocked the LPS-induced phosphorylation and nuclear translocation of nuclear factor ?B (NF-?B). Furthermore, CNMSH attenuated the LPS-induced phosphorylation of extracellular signal-regulated kinase and p38 mitogen activated protein kinase (MAPK), as well as the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway, but did not inhibit the LPS-induced phosphorylation of c-Jun amino terminal kinase.
Conclusions: These results suggest that the inhibitory effect of CNMSH on the LPS-induced production of inflammatory mediators and cytokines in BV2 cells is associated with the suppression of the NF-kB nd PI3KAkt signaling pathways.
|
|
|
KEYWORD
|
|
|
Cheongnoimyungshin-hwan (CNMSH), Microglia cells, Neurodegenerative diseases, NF- kB, PI3KAkt
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|